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Objective To investigate the expression of gene Fmr1 in rats cortex, hippocampus and hypothalamus areas after the rapid eyes movement ( REM ) sleep deprivation .Methods Using the modified multiple platform method (MMPM), 126 rats were randomly and averagely divided into three groups , the normal control group ( CC), the environmental control group (TC) and the sleep deprivation group (SD).Each group was detected on day 1, day 2, day 3, day 5, day 7, and day 9, and the sample tissues were extracted from 7 rats at each time point.Immunohistochemistry and RT-PCR were operated to analysis the expression of gene Fmr 1.Results The expressions of gene Fmr1 were increased gradually in the cortex and thalamus of the SD group after 3 days ( P 0.05).The expressions of gene Fmr1 were decreased gradually in hippocampus for SD after 3 days ( P 0.05 ) . Conclusion The expressions of gene Fmr 1 were increased gradually in the cortex and thalamus but decreased in the hippocampus in the SD group after 3 days.
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Objective To investigate the timing and security of oral glucose tolerance test (OGTT) in patients with acute coronary syndrome (ACS).Methods Two hundred and thirty-two hospitalized patients with ACS were collected from Jan.2007 to Jan.2012.OGTT was undergone in a stable condition at 3 months after being discharged,noting ECG and change of symptoms in patients.Results There was no significant difference in test results of proportions of patients with normal blood sugar [40.95％ (95/232) vs.39.91％ (89/215),x2 =0.051,P ＞ 0.05 ],diabetes [ 25.00％ (58/232) vs.22.33％ (48/215),x2 =0.032,P ＞ 0.05 ],impaired glucose tolerance [ 34.05％ (79/232) vs.33.95％ ( 73/215 ),x2 =0.001,P ＞ 0.05 ],and impaired fasting glucose [2.59％ (6/232) vs.2.33％ (5/215),x2 =0.441,P ＞0.05 ] at 3 months after discharge compared with before discharge when patients were in stable conditions.No significant difference was found on ST segment ECG [ (-0.12 ±0.08 ) mV vs.(-0.15 ± 0.12) mV,t =0.23,P =0.85].Adverse symptoms were not found during the OGTT examination.Conclusion Patients with ACS have a high risk of abnormal glucose metabolism.Undergoing OGTT is safe for stable status patients with ACS.OGTT should be conducted as the routine exam.Early detection and appropriate intervention and treatment are necessary for patients with abnormal glucose metabolism.
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ObjectiveTo investigate the risk of cerebral infarction on pat ients with transient ischemicattack (TIA) by combining ABCD2 score and three imaging methods.MethodsFive hundred and seventy-eight patients with TIA visiting our hospital from January 2007 to December 2011 were assessed for the risk of cerebral infarction after TIA by ABCD2 score method,magnetic resonance diffusion weighted imaging (DWI),magnetic resonance angiography (MRA) and carotid artery color Doppler imaging (CDFI).Cases were followed up on 2 d,7 d and 30 d for incidence of cerebral infarction.ResultsCerebral infarction occurred in 37 cases (6.40％),47 cases ( 8.13％ ) and 61 cases ( 10.55％ ) on day 2,day 7 and day 30.The occurrence of cerebral infarction after TIA in high risk group (35 cases ) by single ABCD2 score was found in 15 cases (42.86％ ),18 cases (51.43％) and 21 cases (60.00％) on day 2,day 7 and day 30.Twenty-eight cases (54.90％),31cases (60.78％ ),and 38 cases (74.51％ ) of patients after TIA with ABCD2 ＞ 3 and DWI positivity (51cases) suffered cerebral infarction on day 2,day 7 and day 30.Cerebral infarction occurred in 22 cases (61.11％),24 cases (66.67％ ) and 30 cases (83.33％) of patients with ABCD2 ＞ 3 points and cerebral artery stenosis (36 cases) on day 2,day 7 and day 30 after TIA.The occurrence of cerebral infarction after TIA on day 2,day 7 and day 30 were in 21 cases ( 60.00％ ),23 cases ( 65.71％ ),28 cases ( 80.00％ ) of the patients with ABCD2 ＞ 3 points and the carotid artery atheroma-caused bureaucratic stenosis (35 cases).There were no significant difference in the value of three methods to assess the risk of cerebral infarction in high-risk TIA groups (P ＞ 0.05).ConclusionTIA patients can progress to cerebral infarction in the short term.There are some predictive value by ABCD2 score from 2 to 30 days for cerebral infarction occurring after TIA,but it is only a simple clinical assessment method.ABCD2 score ＞ 3 points must be combined with DWI,MRA,and other tests in high-risk patients to identify the risk of cerebral infarction,thus improving the accuracy of the prediction.The combined evaluation of TIA patients will help the clinicians give better treatment and decision to this group of patients